Th17/Treg细胞功能轴在抑郁性精神压力诱导老年人动脉粥样硬化中的中介效应
作者:
作者单位:

(1.山东第一医科大学临床与基础医学院,山东省济南市250117;2.山东省医学科学院基础医学研究所,山东省济南市250062)

作者简介:

蔡润泽,研究方向为心脑血管病,E-mail:3449426763@qq.com。通信作者张春菊,副教授,研究方向为动脉粥样硬化病理生理机制,E-mail:zhangchunju94@163.com。

通讯作者:

基金项目:

国家自然科学基金项目(81973139);山东省大学生创新创业训练计划项目(S202110439131);山东省自然科学基金项目(ZR2022MH179);山东省医药卫生科技发展计划项目(202103010686)


Mediating effect of Th17/Treg cell functional axis on arteriosclerosis induced by depressive stress in the elderly
Author:
Affiliation:

1.School of Clinical and Basic Medical Sciences, Shandong First Medical University, Jinan, Shandong 250117, China;2.Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong 250062, China)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    目的]探讨Th17/Treg细胞功能轴在抑郁性精神压力诱导老年人动脉粥样硬化中的中介效应。 [方法]2018年5月—2019年8月在山东济南地区入选60岁及以上社区老年受试者539例,采用老年抑郁量表(GDS)评估受试者的抑郁性精神压力。依据GDS得分五分位将受试者分为低抑郁压力组(n=349)和高抑郁压力组(n=190)。采用颈总动脉内膜中膜厚度(CCA-IMT)及颈-股动脉脉搏波传导速度(cfPWV)评估动脉粥样硬化和僵硬度。检测受试者高敏C反应蛋白(hs-CRP)及外周血Th17和Treg细胞频率及其效应因子。 [结果]与低抑郁压力组比较,高抑郁压力组的CCA-IMT、cfPWV、hs-CRP、Th17细胞频率、白细胞介素17(IL-17)、肿瘤坏死因子α(TNF-α)、IL-6、IL-23、维甲酸相关核孤儿受体(RORγt)mRNA表达及Th17/Treg细胞比率分别升高了24.58%、18.22%、51.59%、44.79%、77.60%、55.94%、61.49%、41.13%、72.11%和150.00%(均P<0.001);Treg细胞频率、IL-10、转化生长因子β1(TGF-β1)及叉头框蛋白P3(Foxp3)mRNA表达分别下降了36.53%、32.96%、27.65%和35.35%(均P<0.001)。校正混杂因素后,GDS得分与CCA-IMT、cfPWV、hs-CRP、Th17细胞频率、IL-17、TNF-α、IL-6、IL-23、RORγt mRNA表达水平及Th17/Treg细胞比率呈独立正相关(均P校正<0.001),与Treg细胞频率、IL-10、TGF-β1及Foxp3 mRNA表达水平呈独立负相关(均P校正<0.001)。Hayes Process分析结果显示,hs-CRP、Th17/Treg功能轴相关指标在GDS与CCA-IMT和cfPWV之间具有显著的中介调控作用(均P校正<0.001)。 [结论]抑郁性精神压力是老年人动脉粥样硬化的独立危险因素。慢性炎症反应可能是抑郁性精神压力诱导老年动脉粥样硬化重要的中介调控因子。

    Abstract:

    Aim To explore the mediating effect of Th17/Treg cell functional axis on arteriosclerosis induced by depressive stress in older adults. Methods From May 2018 to August 9,9 older individuals aged 60 and above were enrolled in community in Jinan area, Shandong. Geriatric depression scale (GDS) was used to evaluate depressive stress of the participants. According to the quintile of the GDS scores, participants were divided into low depressive stress group (n=349) and high depressive stress group (n=190). Common carotid intima-media thickness (CCA-IMT) and carotid-femoral pulse wave velocity (cfPWV) were used to assess arteriosclerosis and stiffness. The serum level of high sensitivity C-reactive protein (hs-CRP) and the frequencies of peripheral blood Th17 and Treg cells and relative cytokines were detected. Results Compared with the low depressive stress group, CCA-IMT, cfPWV, Th17 cell frequencies, the levels of hs-CRP, interleukin-17 (IL-17), tumor necrosis factor alpha (TNF-α), IL-6 and IL-23, the expression of retinoic acid receptor-related orphan receptoryγt (RORγt) mRNA, and the ratio of Th17/Treg cells in the high depressive stress group increased by 24.58%, 18.22%, 51.59%, 44.79%, 77.60%, 55.94%, 61.49%, 41.13%, 72.11% and 150.00% respectively; while the Treg cell frequencies, the levels of IL-10 and transforming growth factor β1 (TGF-β1), and the expression of forkhead box protein P3 (Foxp3) mRNA decreased by 36.53%, 32.96%, 27.65% and 35.35% respectively (all P<0.001). After adjusting for confounding factors, GDS score was independently and positively correlated with CCA-IMT, cfPWV, Th17 cell frequencies, hs-CRP, IL-17, TNF-α, IL-6, IL-23, RORγt mRNA expression, and Th17/Treg cells ratio, whereas it was independently negatively correlated with Treg cell frequencies, IL-10, TGF-β1, and Foxp3 mRNA expression (all P<0.001). The results of Hayes Process analysis showed that hs-CRP, Th17/Treg cells ratio, IL-17, and IL-10 played significant mediating roles in the correlations of GDS with CCA-IMT and cfPWV. Conclusions Depressive stress is an independent risk factor for arteriosclerosis in the elderly. Chronic inflammation may be an important mediating effector in the process of arteriosclerosis induced by depressive stress.

    参考文献
    相似文献
    引证文献
引用本文

蔡润泽,曹芳菡,姚艳丽,张华,刘振东,张春菊. Th17/Treg细胞功能轴在抑郁性精神压力诱导老年人动脉粥样硬化中的中介效应[J].中国动脉硬化杂志,2023,(5):404~410.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2022-08-11
  • 最后修改日期:2023-01-09
  • 录用日期:
  • 在线发布日期: 2023-05-19