大蒜素对高脂饮食ApoE-/-小鼠动脉粥样硬化形成的影响
作者:
作者单位:

(南阳市第二人民医院心血管内科, 河南省南阳市 473000)

作者简介:

王喜欢,硕士,主治医师,研究方向为动脉硬化疾病的基础与临床,E-mail为wangxihuanmed@126. com。


Effects and mechanisms of allicin on atherosclerosis in high-fat diet fed mice
Author:
Affiliation:

Deparment of Cardiovasology, Nanyang Second General Hospital, Nanyang, Henan 473000, China)

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    摘要:

    目的 观察大蒜素对高脂饮食诱导的ApoE-/-小鼠巨噬细胞泡沫化及动脉粥样硬化形成的影响,并从清道夫受体调控的角度探讨其相关的作用机制。方法 6周雄性ApoE-/-小鼠随机分为4组:正常饮食组、高脂饮食组、 高脂饮食+低剂量大蒜素组、高脂饮食+高剂量大蒜素组。采用血脂检测试剂盒测定小鼠血脂水平;使用油红O染色法观察小鼠主动脉根部斑块形成面积和腹腔巨噬细胞泡沫化情况;Western blot法检测腹腔巨噬细胞中清道夫受体SR-A和CD36表达以及JNK和p38激酶磷酸化水平。结果 大蒜素可呈剂量依赖性降低高脂饮食小鼠总胆固醇、甘油三酯及低密度脂蛋白胆固醇的水平(P<0.05),并显著减少主动脉根部斑块面积以及腹腔巨噬细胞泡沫化的形成(P<0.05)。Western blot结果显示大蒜素不仅抑制高脂饮食所诱导的腹腔巨噬细胞中SR-A和CD36表达的增加(P<0.05) ,还能够显著减少JNK和p38激酶磷酸化水平。结论 大蒜素减少SR-A和CD36表达,并抑制JNK和p38激酶激活,从而发挥抗泡沫化和动脉粥样硬化的作用。

    Abstract:

    Aim To investigate the effect of Allicin on foam cell formation and atherosclerosis in ApoE-/- mice fed with high-fat diet, and to further explore the possible molecular mechanisms. Methods 6-week ApoE-/- mice were randomly divided into four groups:normal diet, high-fat diet, high-fat diet with low dose of Allicin, high-fat diet with high dose of Allicin. Total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDLC) and high density lipoprotein cholesterol (HDLC) were examined by a commercially available kit. Moreover, atherosclerotic plaque and foam cell formation were analyzed by oil red O, respectively. Further, the expression of SR-A and CD36 and the phosphorylation of JNK and p38 were determined by western blot. Results The level of TC, TG and LDLC in high-fat diet mice were dose-dependently decreased by Allicin(P<0.05). In addition, oil red O staining revealed that Allicin significantly inhibited the increase of atherosclerotic plaque and foam cell formation in high-fat diet mice(P<0.05). Furthermore, administration of Allicin markedly ameliorated high-fat diet induced increase of SR-A and CD36 expression and JNK and p38 phosphorylation in peritoneal macrophages(P<0.05). Conclusion Our findings demonstrate that Allicin ameliorates foam cell formation and atherosclerosis through inhibition of SR-A and CD36 expression and JNK and p38 phosphorylation.

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引用本文

王喜欢,张金华,胡亚南,张洪.大蒜素对高脂饮食ApoE-/-小鼠动脉粥样硬化形成的影响[J].中国动脉硬化杂志,2017,25(2):140~144.

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  • 收稿日期:2016-08-20
  • 最后修改日期:2016-09-27
  • 在线发布日期: 2017-02-08