Aim To investigate the effect on the expression of CD147 in acute myocardial infarction (AMI) patients as microRNA-548z (miR-548z) binding to the single nucleotide polymorphism (SNP) sites rs8259 T / A at CD147 gene 3′untranslated region (UTR). Methods Samples for the study were from 30 patients with AMI and 13 healthy people. Genotype of rs8259 sites on CD147 gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Quantitative polymerase chain reaction (qPCR) was used to detect the relative expression levels of miR-548z and CD147 mRNA in peripheral blood mononuclear cells (PBMC). The expression of CD147 protein in PBMCs was detected by Western Blot. The interaction between miR-548z and rs8259 different alleles was proved by luciferase reporter gene assay. Results The expression of miR-548z in AA and TT AMI patients, as well as in normal people appeared no significant difference (P>0.05). There was no significant difference between AA and TT patients with AMI in the expression levels of CD147 mRNA in PBMCs (P>0.05). The level of CD147 protein in AMI patients with AA genotype was significantly higher than the TT genotype group. The luciferase reporter gene assay showed that mimic miR-548z could does-dependly reduce the relative luciferase activity of constructs carrying the T allele. Conclusion MiR-548z could bind to rs8259 bearing T alleles on the 3′UTR of CD147 gene and negatively regulate the expression of CD147 protein in AMI patients.