Aim To investigate the mechanism of activation of nuclear factor-κB(NF-κB) by advanced glycation end products (AGE) in endothelial cell. Methods Human umbilical vein endothelial cell(ECV304) were stimulated with AGE-human serum albumin(AGE-HAS), the level of IκBα was mesured by Western blot, and the activation of NF-κB was detected by electrophoretic mobility shift assay(EMSA). Results The degradation of IκBα and the activation of NF-κB could be induced by AGE-HSA in time and dose dependent way in ECV304. The activation of NF-κB could be blocked by inhibiting the degradation of IκBα. Conclusions AGE-HSA might activate NF-κB in IκBα degradation dependent in ECV304. This pathobiological effect of advanced glycation end products might contribute to the development of atherosclerosis.