Aim To study the influence of thrombin receptor on the proliferation of rat vascular smooth muscle cells,probe into mechanism of antisense sequence of thrombin receptor restrained the proliferation of rat vascular smooth cells. Methods Take the incoporation of 3H-thymidine as the target to evaluate vascular smooth muscle cells proliferation; Antisense sequence of thrombin receptor restrained the expression of mRNA of thrombin receptorin vascular smooth cells was detected by RT-PCR; Antisense sequence of thrombin receptor restrained the expression of protein of thrombin receptor was detected by Western-blot; The influence of antisense sequence of thrombin receptor on phosphate inositol of vascular smooth cells was detected by incoporation of 3H-inositol. Results Antisense sequence of thrombin receptor restrained vascular smooth muscle cells proliferation marketedly (compared with control p<0.05),restrained expression of mRNA and protein of thrombin receptor marketedly;Also restrained metabolism of phosphate inositol in vascular smooth cells. Conclusion Antisense sequence of thrombin receptor restrained SD rat vascular smooth muscle cells proliferation marketedly; Antisense sequence of thrombin receptor restrained vascular smooth muscle cells proliferation which could arrested gene expression of thrombin receptor,restrained signal trasduction of vascular smooth muscle cells.