血管紧张素(1-7)对血管紧张素Ⅱ诱导心脏成纤维细胞增殖和胶原合成的影响
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Effects of Angiotensin(1-7) on Cell Proliferation and Collagen Synthesis of Cardiac Fibroblasts Induced by Angiotensin Ⅱ
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    摘要:

    目的探讨血管紧张素(1-7)对血管紧张素Ⅱ诱导心脏成纤维细胞增殖和胶原合成的影响。方法分离培养SD仔鼠心脏成纤维细胞,四氮唑盐比色法检测细胞增殖,流式细胞分析技术测定细胞周期,逆转录—聚合酶链式反应测定心脏成纤维细胞Ⅰ、Ⅲ型胶原mRNA的表达。结果①1.0μmol/L血管紧张素Ⅱ作用24h,心脏成纤维细胞的四氮唑蓝比色分析法吸光度值(0.24±0.01)较无血清对照组(0.14±0.01)明显增加(p<0.01);0.001~1.0μmol/L血管紧张素(1-7)和1.0μmol/L血管紧张素Ⅱ共同干预后吸光度值逐渐降低,分别为0.20±0.01、0.19±0.01、0.13±0.01、0.16±0.03,均显著低于血管紧张素Ⅱ组(p<0.01)。②血管紧张素Ⅱ组心脏成纤维细胞的S期百分率(14.0%±0.9%)和增殖指数(23.4±1.8)较对照组(5.4%±0.7%和10.8±2.4)明显增高(p<0.01);0.1μmol/L血管紧张素(1-7)干预后S期百分率(8.5%±0.7%)和增殖指数(16.2±2.0)较血管紧张素Ⅱ组降低(p<0.01)。③血管紧张素Ⅱ刺激后心脏成纤维细胞的Ⅰ、Ⅲ型胶原mRNA表达水平分别为较对照组明显增高(p<0.01);给予0.001~1.0μmol/L血管紧张素(1-7)和1.0μmol/L血管紧张素Ⅱ共同干预后,胶原表达水平浓度依赖性的下降,均显著低于血管紧张素Ⅱ组(p<0.05)。结论血管紧张素(1-7)具有抑制血管紧张素Ⅱ浓度增加引起的心脏成纤维细胞增殖和胶原合成的作用。

    Abstract:

    Aim To investigate the effect of Angiotensin(1-7) (Ang(1-7) on cell proliferation and collagen synthesis of cardiac fibroblasts(CF) induced by Angiotensin Ⅱ (Ang Ⅱ). Methods CF were isolated and cultured from Neonatal SD rats. Cell number and cell cycle were evaluated by MTT assay and flowcytometry technique. Type and Ⅲ collagen mRNA expressions in CF were analyzed by RT-PCR. Results ①1 μmol/L Ang Ⅱ significantly increased absorption value of CF(0.24±0.01)compared with control group (0.14±0.01, p<0.01), 0.001~1.0 μmol/L Ang(1-7) attenuated the absorption value of Ang Ⅱ group in a concentration dependent manner (0.22±0.01, 0.21±0.01, 0.18±0.01 and 0.16±0.01, p<0.01). ②Compared to the control (5.4%±0.7%, 10.8±2.4), cells in S stage (14.0%±0.9%) and proliferatiion index (23.4±1.8), they were markedly increased in 1 μmol/L Ang Ⅱ group (p<0.01). In the presence of 0.1 μmol/L Ang(1-7), cells in S stage (8.5%±0.7%) and the proliferatiion index (16.2±2.0) were significantly lower than that of Ang Ⅱ group (p<0.01). ③mRNA expressions of typeⅠand Ⅲ collagen in Ang Ⅱ group were significantly higher than those of the control (p<0.01), and in the presence of 0.1 μmol/L Ang(1-7), mRNA expressions were reduced in a concentration dependent manner (p<0.05). Conclusion The results indicate that Ang(1-7) could inhibi the proliferation of CF and the synthesis of type Ⅰand Ⅲ collagen.

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齐峰,王先梅,杨丽霞,郭传明,石燕昆,赵颖,王红.血管紧张素(1-7)对血管紧张素Ⅱ诱导心脏成纤维细胞增殖和胶原合成的影响[J].中国动脉硬化杂志,2007,15(5):361~364.

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  • 收稿日期:2006-05-22
  • 最后修改日期:2007-03-10
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