Aim To investigate the effect of lncRNA-BC200 on the inflammation and apoptosis of nerve cells induced by Aβ₂₅-35 and its possible mechanism. Methods The nerve cells PC12 were divided into NC group (conventional cell culture) and 0,0, 40 μmol/L Aβ₂₅-35 groups (cells were treated with 0,0, 40 μmol/L Aβ₂₅-35 for 24 h), and then cell apoptosis was detected by flow cytometry. qRT-PCR method was used to detect the expression of lncRNA-BC200 in cells. The PC12 cells were divided into NC group (cells were routinely cultured), Aβ₂₅-35 group (PC12 cells were intervened with 20 μmol/L Aβ₂₅-35 for 24 h), si-NC+Aβ₂₅-35 group (PC12 cells were transfected with si-NC and then intervened with 20 μmol/L Aβ₂₅-35 for 24 h), si-lncRNA-BC200+Aβ₂₅-35 group (PC12 cells were transfected with si-lncRNA-BC200 and then intervened with 20 μmol/L Aβ₂₅-35 for 24 h) and TNF-α+si-lncRNA-BC200+Aβ₂₅-35 group (PC12 cells were transfected with si-lncRNA-BC200 and then co-intervened with 20 μmol/L Aβ₂₅-35 and 20 μg/L tumor necrosis factor (TNF-α) for 24 h). Flow cytometry was used to detect cell proliferation activity and apoptosis, ELISA was used to detect the expression of TNF-α, interleukin-6 (IL-6) and interferon-γ (IFN-γ) in the cell culture supernatant, and Western blot was used to detect the protein expression of cleaved-Caspase-3, p-p65 and p-IκBα in the cells. Results The apoptosis rate of PC12 cells and the expression of lncRNA-BC200 were higher in 0,0, 40 μmol/L Aβ₂₅-35 groups than NC group. The protein expression of cleaved-Caspase-3, p-p65 and p-IκBα and the levels of TNF-α, IL-6 and IFN-γ in Aβ₂₅-35 group cells were all higher than NC group. The apoptotic rate of PC12 cells, the protein expression of cleaved-Caspase-3, p-p65 and p-IκBα, and the levels of TNF-α, IL-6 and IFN-γ were all lower in the si-lncRNA-BC200+Aβ₂₅-35 group than Aβ₂₅-35 group. The apoptotic rate of PC12 cells, the protein expression of cleaved-Caspase-3, p-p65 and p-IκBα, and the levels of TNF-α, IL-6 and IFN-γ in the TNF-α+si-lncRNA-BC200+Aβ₂₅-35 group were all higher than the si-lncRNA-BC200+Aβ₂₅-35 group. Conclusion Knockdown of lncRNA-BC200 may inhibit Aβ₂₅-35-induced neuronal cell PC12 secretion of inflammatory factors and apoptosis by inhibiting the activation of NF-κB signaling pathway.