Aim To investigate the correlation among cardiac function indexes left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular ejection fraction (LVEF), A/E peak ratio (A/E), interventricular septum thickness (IVST), left atrium diameter (LAD) and serum biochemical indexes ischemia modified albumin (IMA), homocysteine (Hcy), C-reactive protein (CRP), interleukin-6 (IL-6) in patients with coronary heart disease and myocardial ischemia. Methods 105 patients with coronary heart disease were randomly selected from May 2017 to May 2019 in our hospital. According to the degree of myocardial ischemia, they were divided into angina pectoris group (n＝50) and myocardial infarction group (n＝55). Another 50 healthy people who came to the hospital for physical examination in the same period were selected as the control group. The cardiac function indexes of the three groups were detected by color echocardiography, the levels of serum IMA and Hcy were measured by automatic biochemical analyzer, and the levels of serum CRP and IL-6 were measured by enzyme-linked immunosorbent assay. The relationship between cardiac function index and serum biochemical index was analyzed. Results LVEDD, LVESD, A/E, IVST, LAD of angina pectoris group and myocardial infarction group were significantly higher than those of control group, and LVEF was lower than that of control group; LVEDD, LVESD, A/E, IVST, LAD of myocardial infarction group were significantly higher than those of angina pectoris group, and LVEF was lower than that of angina pectoris group; there were significant differences in cardiac function indexes in pairwise comparison among groups (P＜0.05). The levels of IMA, Hcy, CRP and IL-6 in angina pectoris group and myocardial infarction group were significantly higher than those in control group; IMA, Hcy, CRP and IL-6 in myocardial infarction group were significantly higher than those in angina pectoris group; there were significant differences in serum biochemical indexes in pairwise comparison among groups (P＜0.05). LVEDD, LVESD, A/E, IVST, LAD were positively correlated with IMA, Hcy, CRP and IL-6, while LVEF was negatively correlated with IMA, Hcy, CRP and IL-6 in patients with coronary heart disease and myocardial ischemia (P＜0.05). Conclusion With the increase of serum IMA, Hcy, CRP and IL-6 levels, LVEDD, LVESD, A/E, IVST, LAD increase, while LVEF decrease, suggesting myocardial ischemia in patients with coronary heart disease.