Aim To investigate the expression and correlation of nuclear factor κB (NF-κB), Toll-like receptors (TLR) and inflammatory cytokines in the rupture of intracranial aneurysm (IA). Methods The IA and IA ruptured aneurysm tissues were collected by surgery as the IA group and the IA rupture group, and healthy vascular tissues were collected as a control group. The levels of NF-κB, TLR4, interleukin 1β (IL-1β) and hypersensitive C-reactive protein (hs-CRP) mRNA in aneurysm tissues were detected by qPCR. NF-κB and TLR4 protein levels were detected by Western blot. The levels of IL-1β and hs-CRP in the serum of each group were detected by enzyme-linked immunosorbent assay.Results The levels of NF-κB, TLR4, IL-1β and hs-CRP mRNA and protein in the aneurysm of IA group and IA rupture group were significantly higher than those of the control group (P＜0.05). The levels of NF-κB, TLR4, IL-1β and hs-CRP mRNA and protein in the aneurysm of IA rupture group were significantly higher than those of IA group (P＜0.05). Serum IL-1β and hs-CRP were significantly higher in the IA group and the IA rupture group than in the control group (P＜0.05). The levels of serum IL-1β and hs-CRP in the IA rupture group were significantly higher than those in the IA group (P＜0.05). Conclusion Compared with IA patients, IA-ruptured patients have higher levels of NF-κB, TLR4, IL-1β, and hs-CRP mRNA and protein expression in aneurysms, suggesting that NF-κB, TLR4, IL-1β, and hs-CRP are involved in IA rupture.