Aim To study the clinical characteristics and the time of nucleic acid conversion to negative in patients with Corona Virus Disease 2019 (COVID-19). Methods This study is a retrospective study. Patients with laboratory-confirmed COVID-19 from a hospital in Wuhan from February 2,0 to February 9,0 were divided into mild and common types, followed up to March 0,0, the clinical characteristics and nucleic acid test results of the patients were analyzed. Results A total of 75 patients were included in this study, with an average age of 41.2 ± 11.5 years, and 52.0% were male. Among the patients, 44.0% had fever and 54.7% had positive CT images. The positive rate of the first nucleic acid detection was 69.3%, and the median time of nucleic acid conversion to negative was 9.0 days (6.0~14.0 days). Follow-up to March 0,0, no patients became severe or died. There were no significant differences in age and white blood cell count between patients with mild type (34 cases) and patients with common type (41 cases). Compared with the common group, women in the wild group accounted for 61.8%, which was significantly higher than men (P＜0.05); there was no statistical difference in the time required for nucleic acid conversion to negative between the two groups (8.5 (7.0~13.8) vs 9.0 (5.0~14.0), P＝0.973). The subgroup analysis found that in mild patients, women had less nucleic acid conversion time than men (P＝0.020), and the Lianhuaqingwen capsule subgroup had less nucleic acid conversion time (P＝0.026). Conclusions There was no significant difference in the time of nucleic acid conversion to negative between mild and common patients under the same treatment, and the time of nucleic acid conversion for females and the patients taking Lianhuaqingwen was shortened in mild group. Many patients with moderate COVID-19 may be missed based on clinical manifestations and imaging findings, and accurate and prompt nucleic acid testing or other etiological determination methods are needed.