褪黑素减轻同型半胱氨酸诱导的冠状动脉内皮细胞损伤及机制探讨
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(1.暨南大学医学院附属广州红十字会医院 急诊科,广东省广州市 510220;2.暨南大学医学院附属广州红十字会医院 心内科,广东省广州市 510220)

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关信民,副主任医师,研究方向为心血管内科。通信作者赵强,博士研究生,主任医师,研究方向为心血管疾病。

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广东省科技计划项目(2013B0218033)


Melatonin reduces homocysteine-induced injury of coronary artery endothelial cells and its mechanism
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1.Department of Emergency, Guangzhou, Guangdong 510220, China;2.Department of Cardiology, Guangzhou Red Cross Hospital, Medical College of Jinan University, Guangzhou, Guangdong 510220, China)

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    摘要:

    目的 研究褪黑素(MT)减轻同型半胱氨酸(Hcy)诱导冠状动脉内皮细胞损伤的作用,并探讨其机制是否与激活蛋白激酶B(Akt)/哺乳动物雷帕霉素靶蛋白(mTOR)通路有关。方法 培养人冠状动脉内皮细胞(HCAEC)并分组,对照组用不含药物的DMEM处理,Hcy组用含有1 mmol/L Hcy的DMEM处理,MT组用含有1 mmol/L Hcy 及5 μmol/L MT的DMEM处理,MT+LY组用含有1 mmol/L Hcy、5 μmol/L MT及10 μmol/L LY2942(Akt抑制剂)的DMEM处理。检测细胞活力、细胞凋亡、细胞周期及p-Akt、p-mTOR的含量。结果 Hcy组的细胞活力、S期及G2/M期比例、细胞中Bcl-2、CyclinD1、p-Akt、p-mTOR的含量低于对照组,细胞凋亡率、G0/G1期比例、细胞中Bax、cleaved Caspase-3的含量高于对照组;MT组的细胞活力、S期及G2/M期比例、细胞中Bcl-2、CyclinD1、p-Akt、p-mTOR的含量高于Hcy组,细胞凋亡率、G0/G1期比例、细胞中Bax、cleaved Caspase-3的含量低于Hcy组;MT+LY组的细胞活力、S期及G2/M期比例、细胞中Bcl-2、CyclinD1、p-Akt、p-mTOR的含量低于MT组,细胞凋亡率、G0/G1期比例、细胞中Bax、cleaved Caspase-3的含量高于MT组。结论 MT能够减轻Hcy诱导的HCAEC损伤,调控Akt/mTOR通路是MT减轻HCAEC损伤的相关机制之一。

    Abstract:

    Aim To study the effects of melatonin (MT) on homocysteine (Hcy)-induced injury of coronary artery endothelial cells and clarify whether the mechanism is related to the activation of protein kinase B (Akt)/mammalian rapamycin target protein (mTOR) pathway. Methods Human coronary artery endothelial cells (HCAEC) were cultured and grouped. The control group was treated with DMEM without drugs, the Hcy group with DMEM containing 1 mmol/L Hcy, the MT group with DMEM containing 1 mmol/L Hcy and 5 μmol/L MT, and the MT+LY group with DMEM containing 1 mmol/L Hcy, 5 μmol/L MT and 10 μmol/L LY2942. Cell viability, cell apoptosis, cell cycle and the expression of p-Akt and p-mTOR were detected. Results Cell viability,Sü phase and G2/M phase ratio, the expression of Bcl-2, cyclinD1, p-Akt and p-mTOR in Hcy group were lower than those in control group, apoptotic rate, G0/G1 phase ratio, the expression levels of Bax and cleaved caspase-3 in Hcy group were higher than those in control group; cell viability,Sü phase and G2/M phase ratio, the expression levels of Bcl-2, cyclinD1, p-Akt and p-mTOR in MT group were higher than those in Hcy group, apoptotic rate, G0/G1 phase ratio, the expression levels of Bax and cleaved caspase-3 in MT group were lower than those in Hcy group; cell viability,Sü phase and G2/M phase ratio, the expression of Bcl-2, cyclinD1, p-Akt and p-mTOR in MT+LY group were lower than those in MT group, apoptotic rate, G0/G1 phase ratio, the expression levels of Bax and cleaved caspase-3 in MT+LY group were higher than those in MT group. Conclusion MT can reduce the apoptosis of HCAEC induced by Hcy, regulating Akt/mTOR pathway is one of the mechanisms responsible for MT reducing HCAEC injury.

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关信民,郭壮波,邓鹏程,汤穆浛,朱孟杰,赵强.褪黑素减轻同型半胱氨酸诱导的冠状动脉内皮细胞损伤及机制探讨[J].中国动脉硬化杂志,2020,28(1):25~30.

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  • 收稿日期:2019-04-08
  • 最后修改日期:2019-11-07
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  • 在线发布日期: 2019-12-18