Aim To investigate the effect of carcinoembryonic antigen-related cell adhesion molecule 1 (CC1) on the expression of coxsackie and adenovirus receptor (CAR) and secondary myocardial injury after coxsackievirus B3 (CVB3) infection. Methods The overexpressed mouse CC1 recombinant virus was constructed, and the recombinant lentivirus pLVX-CEACAM 1-ZsGreen-Puro (rLV-CEACAM 1) was packaged and the biological titer of lentivirus was determined. It was divided into CC1 normal cell group, CC1 overexpression group, CC1 normal +CVB3 group and CC1 overexpression+CVB3 group. The apoptosis rate of cardiomyocytes was detected by AnnxinV-PE/ 7-AAD double staining in each group, and cell activity was detected by CCK8. The expression of CAR gene was detected by qPCR. The expression of CAR protein was detected by Western blot, tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β) were measured by ELISA. Results (1)Recombinant vector sequencing CEACAM1 showed a gene sequence connection, which proved mice CEACAM1 recombinant virus vector was built, determination of recombinant lentivirus was 1.5×10¹¹ TU/L. (2)Apoptosis rate of cardiac myocytes in CC1 overexpression+CVB3 group was significantly higher than that in other groups, and the proliferation rate of cardiac myocytes was the lowest (P<0.05). (3)Relative expression of CAR gene was the highest in CC1 overexpression+CVB3 group, and the lowest in CC1 normal group. Relative expression of CVB3 was significantly higher in CC1 overexpression group than in CC1 normal group (P<0.05). (4)Level of TNF-α, IL-1β were higher in CC1 overexpression group, which increased significantly after CVB3 infection. Conclusion CC1 may promote the expression of CAR in cardiac tissue or cell after CVB3 infected cardiac myocytes. CAR might be a potential target for CC1 to regulate the process of cardiac injury caused by CVB3 infection.