Aim To evaluate the value of serum Betatrophin in coronary artery disease of Kazak and Han nationality in Xinjiang. Methods Patients without diabetes mellitus after arteriography were selected as study subjects, including 108 Kazak and 115 Han. The degree of coronary artery lesion was evaluated by Gensini integral system. The patients were divided into two groups according to the Gensini score:the case group and the control group. There were 132 patients in the case group and 91 in the control group. Double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect serum Betatrophin level; AB-ELISA method was used to determine serum ANGPTL3 content; ELISA method was used to detect serum lipoprotein lipase (LPL) content; triglyceride (TG), total cholesterol (TC), fasting blood glucose (FBG) was detected in two groups. Results Compared with the control group, the level of body mass index (BMI), TC, TG, low density lipoprotein cholesterol (LDLC) were increased in the coronary heart disease group (P<0.05) and the BMI, TC, TG, LDLC level in the Kazak coronary heart disease group were higher than that in the Han nationality coronary heart disease group (P<0.05); There was no significant difference in fasting insulin (FINS), FBG, homeostatic model assessment for insulin resistance (HOMA-IR), HOMA-β between the case group and the control group (P>0.05); The serum Betatrophin, angiopioetin-like protein 3 (ANGPTL3), LPL of Kazak patients with coronary artery disease was higher than that of Han patients with coronary artery disease (P<0.05); There was a positive correlation between Gensini score and TC, TG, LDLC, Betatrophin in Kazak coronary artery disease group (r=0.4,0.4,0.2,0.446,P＜0.05); There was a positive correlation between Gensini score and BMI, TC, TG, LDLC, Betatrophin in patients with coronary heart disease in Han nationality (r=0.8,0.5,0.6,0.7,0.393,P＜0.05). Linear regression analysis showed that TG and Kazak were the factors of increasing Betatrophin, regression analysis showed that BMI, LDLC, Betatrophin was the factor influencing coronary artery lesion in Han nationality, and LDLC, Betatrophin, ANGPTL3, LPL was the factor influencing coronary artery disease in Kazak nationality. Conclusion The increase of Betatrophin level may aggravate coronary artery lesion of Kazak and Han nationality in Xinjiang; TG, Kazak may be the factor influencing Betatrophin serological level.