| 孙德春,赵子健,高磊,孙健,杨舸,李晓文,李盛钰.量子降脂仪对高脂血症大鼠血脂的影响及其机制[J].中国动脉硬化杂志,2019,(3):211~215 |
| 量子降脂仪对高脂血症大鼠血脂的影响及其机制 |
| Effect and mechanism of quantum lipid-lowering instrument on blood lipid in rats with hyperlipidemia |
| 投稿时间：2018-09-20 修订日期：2018-12-10 |
| DOI： |
| 中文关键词: 高脂血症 量子降脂仪 胆固醇调节元件结合蛋白2 |
| 英文关键词:hyperlipidemia quantum lipid-lowering instrument SREBP-2 |
| 基金项目:吉林省科技厅项目(201603050YY,20160312019X)；长春市科技局项目(16SS10) |
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| 中文摘要: |
| 目的 观察量子降脂仪对高脂血症模型大鼠的降血脂作用并探讨其机制。方法 以高脂饲料和10%果糖自由饮水建立无特定病原体动物(SPF)级大鼠高脂血症模型。量子降脂仪输出功率强度为70 μW/(cm2·min),每天治疗1~2次,治疗时间为每次5~10 min。采用Elisa试剂盒检测血清中血脂水平,Western blot方法检测脂类代谢和炎症通路关键蛋白表达水平的变化。结果 量子降脂仪明显改善由高脂血症引起的的血脂代谢紊乱、调节血脂平衡、降低炎症状态、缓解肝功能损伤、缓解氧化损伤、提高脂肪代谢相关酶的活性、抑制脂肪酸合成酶的活性。Western blot结果显示,量子降脂仪通过调节核因子κB(NF-κB)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)蛋白的表达水平,缓解机体慢性炎症；通过调节过氧化体增殖物激活型受体α(PPARα)蛋白的表达水平促进机体脂质代谢；通过调节胆固醇调节元件结合蛋白2(SREBP-2)通路,促进低密度脂蛋白受体(LDLR)蛋白表达,缓解脂质代谢紊乱,调节机体高胆固醇和高甘油三酯水平。结论 量子降脂仪具有明显降血脂作用,可作为降血脂医疗器械辅助应用于临床。 |
| 英文摘要: |
| Aim To investigate the effects and mechanisms of quantum lipid-lowering instrument on lipid regulation. Methods High fat diet and 10% fructose were used to establish a rat model of hyperlipidemia. The output power of the quantum lipid-lowering instrument was 70 μW/cm2, and the treatment time was 5~10 minutes for each time,1~2 times a day. Elisa kit was used to detect serum lipid levels and Western blot was used to measure the expressions of key protein in lipid metabolic and inflammatory pathways. Results Quantum lipid-lowering instrument can improve the disorder of blood lipid metabolism, regulate blood lipid balance, reduce inflammation, alleviate liver function damage, alleviate oxidative damage, increase the activity of lipid metabolism-related enzymes, and inhibit the activity of fatty acid synthase which is caused by hyperlipidemia. Western blot showed that Quantum lipid-lowering instrument could alleviate inflammation by regulating the expression levels of nuclear factor κB (NF-κB), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6);modulate the expression of peroxisome proliferators-activated receptors α (PPARα) which could improve lipid metabolism;regulate the sterol-regulatory element binding proteins 2 (SREBP-2) pathway to increase low-density lipoprotein receptor (LDLR) protein expression, alleviate lipid metabolism disorders, and regulate cholesterol and triglyceride levels. Conclusion Quantum lipid-lowering instrument has obvious effect of lipid regulation, and can be used as an assisted clinical instrument for lowering lipid. |
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