Aim To investigate the relationship between uric acid, blood lipids and ambulatory arterial stiffness index (AASI). Methods A total of 280 subjects without taking anti-hypertensive medication from Fuwai Hospital during January to June, 2011 were enrolled. Ambulatory blood pressure monitoring (ABPM) and blood biochemical examination were performed among all subjects. AASI was calculated as 1 minus the regression coefficient of diastolic blood pressure on systolic blood pressure according to ABPM recordings. Pearson correlation analysis and stepwise multivariable linear regression models were used to assess the relationships between AASI and biomarkers. Results Among 280 subjects, 161 were men, 138 were hypertensive, and the average age was (50.4±13.3) years old. Pearson correlation analysis indicated that AASI was related to serum uric acid (r=0.168, P=0.049), total cholesterol (r=0.269, P=0.001), low-density lipoprotein cholesterol (LDLC) (r=0.223, P=0.009) in hypertensive participants. Stepwise multivariable linear regression analysis indicated that AASI was independently associated with serum uric acid (β=0.219, P=0.007) and total cholesterol (β=0.226, P=0.005) in hypertensive participants. Conclusion Serum uric acid and total cholesterol are independently correlated to AASI in hypertensive patients.