Aim To establish systematic methods for studying intravascular lipid metabolism of zebrafish. Methods Oil red O staining, CholEsteryl BODIPY 542/563 C11 fluorescently labelling and blood lipids detecting were used to study intravascular lipid metabolism of embryo, larvae and adult zebrafish and the effect of different diet on blood lipid of zebrafish. Results Intravascular lipid of embryo zebrafish was stained by oil red O staining, and intravascular cholesterol was labeled by CholEsteryl BODIPY 542/563 C11. Increased intravascular lipid of larvae zebrafish incubated in egg yolk was stained by oil red O staining, and increased intravascular cholesterol of larvae zebrafish fed by high cholesterol diet was labeled by CholEsteryl BODIPY 542/563 C11. The serum total cholesterol and triglyceride of adult zebrafish were obviously increased after 7 weeks fat feeding. Conclusion Oil red O staining, CholEsteryl BODIPY 542/563 C11 fluorescently labelling and blood lipids detecting can be applied for zebrafish intravascular lipid metabolism research.