Aim To observe the protective effect of Baicalin on vascular endothelial cells induced by oxidized low density lipoprotein (ox-LDL). Methods In vitro cultured human umbilical vein endothelial cells (EAhy926) were divided into control group, ox-LDL group, Baicalin group and different concentrations of Baicalin (5,0 and 100 mg/L)＋ox-LDL groups, cultured 24 h. Cell viability was detected by CCK-8, ELISA was used to detect the level of IL-6 and TNF-α in cell culture supernatant, Western blot was used to detect the expression of apoptosis related protein Bax and Bcl-2. Results Compared to the ox-LDL group, the cell survival rates of 0,0 mg/L Baicalin＋ox-LDL groups were significantly increased (P＜0.05), the protein levels of both TNF-α and IL-6 in cell culture supernatant were decreased significantly (P＜0.05), and the ratio of Bax/Bcl-2 decreased (P＜ 0.05). Conclusion Baicalin protects against endothelial cell injury, which may be achieved by inhibiting inflammatory factors, reducing apoptosis and enhancing cell activity.