Aim To investigate the effect of different concentration of serum amyloid P component (SAP) on inflammation and oxidative stress and its probable mechanism. Methods RAW264.7 cells were divided into control and SAP group (1.25 mg/L, 2.5 mg/L, 5 mg/L) with different concentrations of SAP co-culturing for 24 hours. Expression of intercellular adhesion molecule 1 (ICAM-1) and Fc gamma receptor (FcγR) were measured by flow cytometry. ELISA and RT-PCR were used to measure inflammatory factors, IL-1β and TNF-α, ICAM-1 and reactive nitric species, nitric oxide (NO). Expression of protein (Spleen tyrosine kinase, Syk, extracellular signal-regulated kinase, ERK1/2 and pERK1/2) related to information was detected by Western blot. Results RAW 264.7 cells were co-cultured with different concentrations of SAP for 24 hours. Secretions of inflammatory factors, IL-1β and TNF-α (P＜0.05), ICAM-1 and NO were significantly increased in SAP groups. However, expressions of FcγR and pERK1/2 were not affected by the treatment of SAP. For the key protein in downstream pathways of FcγR, Syk was dose-dependent increased and downstream pathways was activated. Conclusion SAP increased expression of Syk, promoted secretion of inflammatory factors and oxidative stress after combined with Fcγ receptors.