Notch1 signaling pathway, as an evolutionarily conserved system of signal transduction, plays an important role in cardiovascular development and vascular repair throughout the embryonic period and postnatal day. Vascular calcification results not from a passive deposition of hydroxyapatite on vascular wall, but rather is an active cell-mediated process involving vascular smooth muscle cell(VSMC) transforming into osteoblast-like cells. It can be regulated by multiple factors and many signaling pathway. Recent studies indicate that Notch1 signaling pathway is significantly associated with vascular calcification. In this paper, the composition, effect and physiological function of Notch1 signaling pathway, the mechanism and regulation of vascular calcification, and the relationship between Notch1 signaling pathway and vascular calcification are summarized.