Aim To establish the swine model of myocardial infarction by percutaneous coronary artery embolism with gelatin sponge, and to observe the dynamic changes of early serum ischemia modified albumin （IMA） in swine with acute myocardial infarction （AMI）. Methods 16 Chinese mini-swines were via femoral artery puncture after anesthesia, and obtuse marginal branch was blocked by injecting gelatin sponge via micro catheter after coronary artery angiography. The dynamic changes of IMA were observed by continuously measuring serum IMA in the preoperative and postoperative 10, 30 min and 1, 2, 3, 4, 6, 8, 10, 12, 24, 48 h. The swines were sacrificed at 1, 3, 5, 7, 10 days after the AMI, and specimens of the myocardium were taken for histopathological examination. Results The 15 mini-swine models of myocardial infarction were established successfully, and AMI was confirmed by electrocardiogram, cardiac specimen and histopathological examination. IMA began to rise after 10 min and arrived the highest serum concentration in 6 h, and decreased to normal range in 48 h after AMI. Conclusions The method of swine AMI model established by percutaneous coronary artery embolism with gelatin sponge is scientifically reliable, simple, and repeatable. IMA is sensitive marker in the early of AMI, and dynamic detection of serum IMA level has certain clinical significance in early diagnosis of AMI.