Aim To study the immunization effects of B epitopes of heat shock protein 65 on the atherosclerosis plaque formation of high fat diets animals. Methods By using two functional epitopes P1（179-190）,P2（31-46）of HSP65 related to inflammatory autoimmune disease,high-level expression vector of pET28a-CTB-p117 was built with CTB as a carrier protein.The fusion protein of CTB-p117 was acquired by expression and purification process and used to immunize the animals by subcutaneous and intracutaneous injections. Results Anti-HSP65 antibodies were induced and both blood total cholesterol levels and aortic As plaques significantly increased compared with control group （P<0.05）.Conclusions The HSP65 epitopes （CTB as the carrier protein） were able to aggravate As plaque formation after subcutaneous injections which could be the reasons that the induced anti-HSP65 antibodies promoted inflammatory reaction,lipid metabolic changes,and then accelerate the As plaque formation.The above HSP65 epitopes can be used to construct the anti-As vaccine to inhibit the As plaque formation by different immunization way.