Aim To investigate the effects of rosuvastatin on superoxide dismutase （SOD） and malondialdehyde （MDA） in human umbilical vein endothelial cells（HUVEC） injured by homocysteine （Hcy）. Methods The HUVEC were primary cultured. HUVEC from the second to third generations were stimulated with different concentrations of Hcy and model of cell injury was set up. Then the HUVEC were pretreated with rosuvastatin in different concentration. Real-time PCR was used to detect the expression of SOD1 mRNA. Activity of SOD was detected by xanthine oxidase technique. The content of MDA was determined by TBA method. The level of nitric oxide （NO） in culture cell supernate of HUVEC was measured by nitrate reductase method using NO assay kit. Results Compared with control group, the expression level of SOD1 mRNA and the content of NO and SOD was significantly decreased, the content of MDA was significantly increased after 24 hours in Hcy group （P<0.05）, while pretreated with rosuvastatin for 2 hours, the expression level of SOD1 mRNA and the content of NO and SOD was significantly increased, the level of MDA was significantly decreased （P<0.05）. Conclusion Rosuvastatin shows its anti-oxidative effect through improving SOD activity and reducing the level of MDA in HUVEC injured by Hcy, and the effect is independent on its lipid-lowering effect.