Aim To regulate the expression of matrix metalloproteinase inducer （CD147） from Jurkat T cells,and observe its influence on monocyte chemotaxis. Methods Jurkat T cells were stimulated with cyclophilin A （CypA） of different concentrations,and the expression of CD147 protein from Jurkat T cells was determined with Western blot. Jurkat T cells were transfected with CD147-targeting small interfering RNA （siRNA） through the carrier Lipofectamine^TM2000 for 48 hours. The expression variation of CD147 mRNA and protein were determined by RT-PCR and Western blot respectively. The expression of CD147 from Jurkat T cells was up-regulated with CypA and down-regulated with CD147-targeting siRNA,then these Jurkat T cells and THP-1 monocytes were co-cultured in transwell assay to count the number of migrated THP-1 cells. Results Compared with blank control group,the expression of CD147 protein from Jurkat T cells increased along with the concentration gradient of CypA in the range of 0 to 400 μg/L （P<0.05）. Compared with the 400 μg/L CypA group,the expression of CD147 protein was not different with that of the 800 μg/L Cy-pA group （P＞0.05）. Compared with negative control siRNA,CD147 mRNA and protein levels decreased in Jurkat T cells transfected with CD147-targeting siRNA,and the inhibition ratios were 76.27%±2.00% and 71.67%±4.70% （P<0.05）. In the coculture system of Jurkat T cells pretreated with CypA and THP-1 cells,the number of migrated THP-1 cells （196.00±9.88 Cells/Field） significantly increased,compared with blank control group （109.00±8.22 Cells/Field P<0.05） however,in the coculture system of Jurkat T cells pretreated with siRNA and THP-1 cells,the number of migrated monocytes （44.00±6.98 Cells/Field） significantly decreased,compared with blank control group （109.00±8.22 Cells/Field P<0.05）. Conclusions CD147 expressed by Jurkat T cells was able to promote the chemotaxis of monocytes in coculture system.