Aim To investigate the regulating effects of P38MAPK signaling pathway on the differentiation of the bone marrow-derived stem cells（BMSC） induced by BMP-2 toward cardiomyocyte-like cells and to determine the possible mechanisms. Methods BMSC were separated and cultured in vitro. The third passage cells were divided into 3 groups: control group, BMP-2 inducer group,BMP-2+P38MAPK blocker SB203580 group. The morphological observation was performed by the inverted phase contrast microscope, the immunohistochemical technique was used for detecting the expression of cardiac specific troponin（cTnT） and connexin 43（Cx43）,Western blot was used for examining of the changes in p-P38MAPK/P38MAPK levels in BMSC after induced with the BMP-2. Results Expression of p-P38MAPK of BMSC could be observed at 15 min after induction with BMP-2, increased obviously at 30 min,increased increasingly from 60 min. Compared with inducer group,the expression of p-P38MAPK of blocker group decreased obviously（P<0.05）. The expression of cardiac specific troponin（cTnT） and Cx43 were negative in control group, and positive in inducer group and blocker group. Compared with inducer group, Cx43 positive cells increased in blocker group（P<0.05）. Conclusion BMP-2 could induce differentiation of BMSC toward cardiomyocyte-like cells,and the P38MAPK sinaling pathway plays a negative regulating role during induction.