Aim To investigate angiotensin Ⅱ （Ang Ⅱ） and oxidized low density lipoprotein （ox-LDL） regulating the expression of Smurf2 and collagenⅠin cultured rat vascular smooth muscle cells （VSMC）. Methods The mRNA expression was tested by RT-PCR. The protein expression was examined by Western blot. The protein expression of collagenⅠwas examined by ELISA. Results Compared with control group, Ang Ⅱ decreased Smurf2 expression （P<0.05） and enhanced collagenⅠexpression （P<0.05）, ox-LDL enhanced Smurf2 expression （P<0.01） and decreased collagenⅠexpression （P<0.05）. Ox-LDL concentration-dependently induced Ang Ⅱ-decreased Smurf2 expression （P<0.05） and collagenⅠ concentration. Conclusion Ang Ⅱ decreased Smurf2 expression in cultured VSMC. Ang Ⅱ may inhibit Smurf2 expression to improve collagenⅠ production. ox-LDL enhanced Smurf2 expression. ox-LDL may improve the expression of Smurf2 to suppress the production of collagenⅠ. ox-LDL concentration-dependently induced Ang Ⅱ-decreased Smurf2 expression, while decreased collagenⅠ expression.