AimTo investigate the effect of telmisartan on the expression of vascular cell adhesion molecule-1(VCAM-1), Nuclear factor-κB（NF-κB）induced by homocysteine (Hcy) in human umbilical vein endothelial cells（HUVEC）and the adhesion to monocytes.MethodsCollagenase was used to isolate the endothelial cell from human umbilical vein.RT-PCR was used to examine the expression of VCAM-1 mRNA.Western blot was used to detect expression of VCAM-1 and NF-κBp65 protein.ROSE BENGAL staining technique was used to detect Mononuclear cells-endothelial cell adhesion function.ResultsThe expression of VCAM-1 mRNA，VCAM-1 protein，NF-κB p65 protein and the adhesion function to monocytes were induced by Hcy in HUVEC comparaed with the control group （p<0.01）.By comparion with the Hcy group, telmisartan (1000 nmol/L) obviously decreased the expression of VCAM-1 mRNA，VCAM-1 protein，NF-κB p65 protein and the level of endothelial cells adhesion to monocytes induced by Hcy in HUVEC（p<0.01）.NF-κB inhibitor remarkably antagonized the expression of NF-κB p65 protein， VCAM-1 mRNA and VCAM-1 protein induced by Hcy in HUVEC，and the adhesion function between mononuclear cells and endothelial cells was also decreased（p<0.01）.ConclusionTelmisartan decreased the higher expression of VCAM-1 mRNA, protein and the level of endothelial adhesion with monocytes induced by Hcy in HUVEC.The mechanism may be through inhibiting the activation of nuclear factor-κB to control inflammation and endothelial injury.