AimTo construct PGL3-ApoM luciferase reporter vector containing ApoM gene regulation area, and to investigate the effect of C→T substitution in ApoM promoter －778 bp region on ApoM gene expression.MethodsHuman chromosome DNA fragments containing ApoM gene were amplified by PCR, and the DNA fragments consisting of ApoM TT and CC genetypes were separately selected, then PGL3 vector including above two different DNA fragments was constructed.Recombinant vector were contransfected into HepG2 cells by using cationic liposome method.Cells were cultured for 48 h, activity of firefly luciferase was measured.ResultsRelative activity of luciferase for ApoM CC genetype was significantly lower than that for TT genetype.Conclusion－778 bp C→T substitution in ApoM gene may inhibit ApoM gene transcription.It may be the important factor of the ApoM gene expression.