AimTo Explore influence of anti-cardiolipin antibodies (ACA) and anti-neutrophil cytoplasmic autoantibodies (ANCA) on intima membrane thickness (IMT) and carotid artery stenosis of patients with acute cerebral infarction(ACI).Methods250 cases of the first onset ACI patients were continously collected, enzyme-linked immunosorbent assay was used to determine ACA in morning hollow serum of ACI patients, indirect immunofluorescence was used to determine ANCA.All selected patients were divided into three groups: the ACA positive group, ANCA positive group, negative control group (ACA and ANCA negative).Carotid artery was detect by ultrasonic, IMT and carotid artery stenosis degree were compared in three groups.ResultsLeft and right side IMT of ACA positive group and ANCA positive group were higher than negative control group in patients with ACI (p<0.001); Extracranial carotid artery stenosis rate in ACA positive group(88.68%) and ANCA positive group(85.71%) were higher than negative control group(54.71%,p<0.017).ConclusionACA, ANCA were related to IMT, carotid artery stenosis in ACI patients.