Aim To observe effect of lectin-like oxidized low density lipoprotein(ox-LDL) receptor-1(LOX-1) gene silence on the expressions of fractalkine and monocyte chemoattractant protein-1(MCP-1) induced by ox-LDL in human umbilical vein endothelial cells(HUVEC).Methods HUVEC were cultured and treated with ox-LDL of three different concentrations(25,50,100 mg/L respectively) for 24 hours;pGenesil-1 LOX-1 shRNA were transfected into HUVEC and then cells were treated with ox-LDL(50 mg/L).mRNA and protein expressions of LOX-1,fractalkine and MCP-1 were determined by semi-quantitative reverse-transcription polymerase chain reaction(RT-PCR),Western Blot analysis and enzyme linked immunosorbent assay.Results Ox-LDL could induce mRNA and protein expressions of LOX-1,fractalkine and MCP-1 in a dose-dependent manner(P<0.01).Expressions of fractalkine and MCP-1 induced by ox-LDL(50 mg/L) were significantly suppressed by transfection with LOX-1 specific shRNA(P<0.01).Conclusions Ox-LDL can induce expressions of fractalkine and MCP-1 in a dose-dependent manner in HUVEC,and this process can be suppressed by LOX-1 gene silence.