Aim To study the effect of transfection of adenovirus-mediated integrin-linked kinase(ILK) on rat bone marrow mesenchymal stem cell(BMSC) paracrine action.Methods BMSC were isolated and cultured in vitro by adherent culture.The recombinant adenoviral vector was constructed containing both human wild-type ILK cDNA and humanized recombinant green fluorescent protein(hrGFP).Corresponding virus with null content(adeno-null) was used as a control.BMSC were infected by adeno-ILK(MSC-ILK group) or adeno-null(MSC group) in serum-free medium.The infection efficiency of adeno-ILK was tested by GFP expression using flow cytometry,thus the best multiplicity of infection(MOI) was identified.The mRNA was extracted from BMSC and the gene expression of vascular endothelial growth factor(VEGF),fibroblast growth factor-2(FGF-2),and insulin-like growth factor-1(IGF-1) was examined by Real-time PCR.Results The gene expression of VEGF,FGF-2,and IGF-1 in the MSC-ILK group was 8.2±0.4,2.6±0.2 and 2.4±0.2 times(P<0.05 or P<0.01) higher than that in the MSC group respectively.Conclusion BMSC transfected by ILK gene could express higher VEGF,FGF-2,and IGF-1.