Aim To observe the association of fibrinogen β148C/T gene polymorphism with plasma fibrinogen levels and cerebral infarction(CI) in Hunan Han population. Methods 150 subjects with CI and 111 controls were enrolled in the study.The beta fibrinogen site C148T was genotyped by using restriction fragment length polymorphism method.The level of plasma fibrinogen was measured by the Clauss method. Results The frequencies of T allele was 0.325 in CI group,and 0.200 in control group,frequency of T allele was significantly higher in CI group than that in control group(P>=0.041).Furthermore,the results of Logistic regression analysis showed that the genotype of TT was an independent risk factor for CI(Or=2.040,95%CI was 1.283~3.243,P>=0.003).Plasma fibrinogen levels were higher in CI subtypes than those in control subtypes.In both CI group and control group,plasma fibrinogen levels in TT genotype were higher than those in CC and CT genotype. Conclusion Fibrinogen β148C/T polymorphism may be associated with susceptibility to CI in Hunan Han population.TT genotype increased risk to CI.Fibrinogen β148C/T gene polymorphism may have association with plasma fibrinogen level.Carriers of TT genotype at fibrinogen β148C/T gene polymorphism have higher levels of plasma fibrinogen.