Aim To construct vein grafts restenosis model in the C57BL/6 mouse to mimic graft restenosis after coronary artery bypass grafting. Methods Under anesthesia,the inferior Vena cava in mice was harvested and grafted into the carotid artery in "non-suturing sleeve pattern" with a cuff or in interrupted stich.Patency of grafts was evaluated respectively.Animals were sacrificed at the 1 week,2 weeks,4 weeks and 6 weeks postoperation,and the grafted tissue fragments were stained with HE and immunohistochemically. Results In the experimental group 1 mouse died due to overdosage of pentobarbital,all grafts were patent without distortion and leakage immediately after procedure and 2 grafts failed due to thrombosis,the average operation time was 46.0±10.6 min.In the control group 2 mice died from hemorrhage immediately after procedure,2 grafts failed due to thrombosis,the average operation time was 68.0±12.4 min.Vessel wall thickening was observed and progressed gradually at the time of harvest,which resulted in growing narrow of graft lumen.Histological analysis demonstrated varying degrees of neointimal hyperplasia and inflammatory cells. Conclusion The inferior Vena cava vein graft model established by using sleeving,which is more efficient and less leaky with higher graft patency when compared to interrupted stich,may be an ideal model for further study on the prevention of vein intimal hyperplasia after bypass grafting.