Aim To investigate the expression discipline of Survivin after perifocal areas of cerebra after ischemia-reperfusion(I/R) in rats,and to identify the effect of ifenprodil on Survivin after I/R. Methods Seventy-two Wistar rats were randomly divided into sham group,model group and ifenprodil group.The right middle cerebral artery occlusion reperfusion(MCAO/R) model was established by thread ligation.After operation in sham group,2 hours ischemia of middle cerebral artery was followed with 3 hours,24 hours,72 hours and 7days reperfusion in model group and ifenprodil group.Rats were sacrificed and Survivin in hippocampus of ischemia side was detected by immunofluorescence. Results Fluorescence intensity of the sham group was very weak,showing the basal expression;while fluorescence intensity of model group and ifenprodil group had almost the same trend,which expression rose 3 hours after I/R,increased continuously and reached the peak at 72 hours,and then decreased afterward,and there were significant differences compared with the sham group(P><0.05).Fluorescence intensity of ifenprodil group were stronger than the model group at the same time point(P><0.05). Conclusion Expression of Survivin increased after MCAO/R,reaching the maximum at 72 hours.Ifenprodil had a protective effect on the damage of I/R.