Aim To explore the effects of advanced oxidation protein products(AOPP)on expressions of stromal cell-derived factor-1α(SDF-1α)in ECV304 cells and the signal pathway that mediated the effects.Methods AOPP-BSA was made from bovine serum albumin(BSA)and sodium hypochlorite.After different time treated with AOPP-BSA,the expressions of SDF-1α mRNA in ECV304 cells were measured by reverse transcription polymerase chain reaction(RT-PCR)and the expressions of SDF-1α protein and the levels of phosphored-p38 mitogen-activated protein kinase(MAPK)were analyzed by Western Blotting.In inhibition test SB₂03580,the special inhibitor of p38MAPK of different concentrations were added into ECV304 culture media for 1 hour,then the cells were treated with AOPP-BSA for 12 hours,at last the protein levels in supernatant were measured by enzyme linked immunosorbent assay(ELISlA).Results Compared with control group,the expressions of SDF-1α mRNA and protein in ECV304 cells increased significantly after incubated with 200 μmol/L AOPP-BSA for 2 hours(P><0.05).The expression of SDF-1α mRNA peaked after 6 hours(P><0.01)and decreased gradually until there was no differentiation at 24 hours.Expression of SDF-1α protein increased in a time-dependent manner and peaked at 24 hours(P><0.01).After 15 minutes the levels of phosphored-p38MAPK increased significantly(P><0.05).When the p38MAPK pathway was blocked by SB₂03580(0.1 μmol/L,1 μmol/L,10 μmol/L),the promoting effects of AOPP-BSA on expressions of SDF-1α protein in ECV304 cells were significantly inhibited(618.85±60.12,500.98±69.47,359.97±59.81,P><0.05 or 0.01).Conclusion AOPP induced the expression of SDF-1α from ECV304 cells,p38MAPK signal pathway is an important pathway that mediated the effects.