Aim To observe the effect of ghrelin on human umbilicus endothelial cell(HUVEC) proliferation and migration. Methods Different concerntration of angiotesionⅡ(AngⅡ)(10-9～10-6 mol/L) was adopted to excite HUVEC for 24 hours,and the best concerntration of 10-6 mol/L was acquired,and used to stimulate HUVEC for 0,6,12,24 hours respectively to observe human umbilicus endothelial cell proliferation and migration.(10-9～10-6 mol/L) ghrelin was pretreated for 2 hours and cultivated for 24 hours with HUVEC and 10-6 mol/L ghrelin was used to pretreat for 0,0.5,1,2 hour and cultivate HUVEC for 24 hours to observe the cells proliferation and migration.MAPK /ERK1/2 singal pathy inhibitor PD98059(25 μmol/L),GHSR1a receptor inhibitor(GHRP-6 25 μmol/L) were added to pretreat HUVEC to observe ghrelin effect on AngⅡ induced endothelial cell injury.Cell proliferation was measured with WST-8,and cell migration with Transwell;ERK1/2 and p-ERK1/2 protein expression with western blot. Results AngⅡ promoted HUVEC proliferation and migration with dose and time dependent manner.Ghrelin inhibited AngⅡpromoting effect on cell migration and proliferation with dose and time dependent manner.Pretreatment with PD98059(25 μmol/L) can inhibit the promoting effect of HUVEC proliferation and migration;pretreatment with GHRP-6(25 μmol/L) can block ghrelin's inhibiting effect on cell migration and proliferation effect.Ghrelin can decrease the p-ERK1/2 expression. Conclusion Ghrelin can inhibit AngⅡinduced HUVEC proliferation and migration,and the mechanism was involved in ERK1/2 pathway.