Aim To analyse bininformatics on heart developmental candidate gene ZNF569 providing guiding opinion for functional study of ZNF569. Methods Bininformatical analysis on ZNF569 was made by utilizing Bininformatical Web and soft ware. Results Protein containing KRAB/C2H2 is an important transcriptional factor.Alignment between the cDNA sequence and human genome indicates that ZNF569 is identical on chromosome 19q13.12,spanning approximately 56.28 kb in reverse manner on the genome and organized into 6 exons ZNF569 encodes a putative protein of 686 amino acids.The predicted amino acid sequence of ZNF569 contains an N-terminal Krüppel-associated box(KRAB) domain,and a series of 18 C2H2 zinc finger motifs that extend to the end of the protein sequence.A search of published DNA databases for sequences similar to that of ZNF569 demonstrated that they had varying degrees of similarity to a number of previously identified C2H2-type zinc-fingers protein. Sequence analysis and database comparison indicate that the predicted protein contains 18 different C2H2 zinc-finger domains in tandem arrays,characteristic of transcription factor proteins of this family. Conclusion A search of analyzed evolutionary relationship among different zinc-finger proteins with phylogenetic tree analysis show that ZNF569 is one of the conserved proteins during evolution.