Aim To examine the effect of timisartan on expression and activity in vascular remodeling after artery injury in rats. Methods The rat models of balloon endothelial denudation were builded. Male Wistar rats were randomized to three groups: nomal control group (n=12), model group (n=12) and timisartan group (n=12). The rats in timisartan group were supple diet with timisartan 40 mg/(kg·d). The animals were sacrificed at 30 days after the injury. Western blot were used to detect the expression and activity of focal adhesion kinase (FAK) with RT-PCR or Western blot method. Hyperplasia of intima were observed. Results Compared with the control group, hyperplasia of intima were significantly increased, the expression of FAK mRNA, protein and its activity were significantly increased in model group at 30 days after the injury. Compared with the model group, hyperplasia of intima were significantly lower, the expression of FAK mRNA, the expression of FAK protein and activity were significantly decreased inTimisartan group. Conclusion Timisartan significantly inhibited artery wall remodelling and expression and activity of FAK in the rat model of artery injury. The mechanisms of Timisartan inhibiting artery wall remodelling could involve inhibition of expression and activity of FAK.