Aim To study the effect of advanced glycation end products(AGE) and nitric oxide(NO) on type 2 diabetes and chronic complications of diabetes,and the correlation between AGE and NO. Methods The levels of serum AGE and NO were detected by flow injection assay and spectrophotometry. Results The level of serum AGE in all patient groups was significantly higher than that in control group, and the NO level was significantly lower than that in control group(all p<(0.01)). Conclusion The increase of serum AGE played an important role in mediating the formation of diabetes,chronic complications of diabetes,cardiovascular and cerebrovascular diseases,and the reduction of serum NO level was probably one of reasons which resulted in vascular disfunction and atherosclerosis.The increase of serum AGE may not be an independent influential factor for the reduction of serum NO.