Aim To study the effect of levo-anlodipine on lactate dehydrogenase (LDH) and cardiac troponin of the anoxic myocardial cells. Methods Rat myocardial cells were cultured and randomly divided into normal group, anoxic injured group and levo-anlodipine group. Then the anoxic model was set up. After that, the concentrations of LDH, cardiac troponin Ⅰ (cTnⅠ) and dissociative calcium of myocardial cells (with Fura2/am fluorescent probe) were measured in each group. Results The concentrations of LDH, cTnⅠ and dissociative calcium of anoxic group were obviously higher than those of the control group (p<0.01)and levo-anlodipine can lessen the concentrations of LDH, cTnⅠ and dissociative calcium (p<0.01). Conclusion Levo-anlodipine can protect the anoxic myocardial cells by attenuating the calcium overload.