Aim To investigate the relationship between total white blood cell (WBC) Count with cardiovascular risk factors in healthy subjects. Methods The age,gender and history of smoking of 138 subjects were investigated. The body mass index (BMI),waist circumference (WC), waist to hip ratio (WHR),systolic blood pressure (SBP),Diastolic blood pressure (DBP),triglyceride (TG),total cholesterol (TC),low density lipoprotein,high density lipoprotein- cholesterol (HDLC),fasting insulin,and tumour necrosis factor-α (TNF-α) were also determined. A 75 g oral glucose tolerance test was performed in all participants. Insulin resistance was evaluated by using Homeostasis Model Assessment (HOMA). Results Total WBC count was significantly positive correlation(p<0.05) with BMI,WC,WHR,SBP,DBP,TNF-α,2 h glucose after glucose load,LnTG,LnFIns,and Ln(HOMA-IRI) (all p<0.05) and negative correlation with HDLC (P=0.006). In a multiple stepwise regression analysis,only WC,TNF-α and history of smoking were positive predictors of total WBC count after adjusting for all potentially confounding variables. Conclusion The total WBC count associates with cardiovascular risk factors such as obesity,2-h glucose after load,blood pressure,TG,HDL-C and history of smoking.