Aim To prepare the polyclonal antibodies against VLDL receptor and to characterize the antibodies. Methods Rabbits were immunized with polypeptide fragment of VLDL receptor as antigen. The blood serum of the immunized rabbits was collected. The quality of the antibodies were analyzed and characterized with ELISA and Western Blot. Results Rabbit against mouse and human VLDL receptor antibodies were obtained. The anti-serum have high title and can recognize the two subtypes of natural VLDL receptor through Western Blot. Conclusions The polyclonal antibodies against VLDL receptor were obtained, which provide a new tool to study the protein of VLDL receptor.