Aim The study aims to observe how lipoprotein lipase activator NO-1886 affect the content of vanadium and chromium in pig pancreas with high fat and high sugar. Methods Guizhou minipigs were divided into three groups randomly: control group fed with basic feedstuff, sugar fat group of fed with high fat and high sugar feedstuff, and NO-1886 group fed with high fat and high sugar feedstuff in the first 3 mouths and then added 1 % NO-1886 since the 4th mouth. The pigs were fed separately. Theirs sugar, fat and insulin of blood plasma were observed. The pigs were killed to get the pancreas at the end of the experiment and the tissue were digested with acid. The content of vanadium and chromium were tested Atomic Emission Spec trometry. Resutls The sugar fat group and NO-1886 group are as the followings: before the NO-1886 was added, the blood insulin in each group is 11.4±2.7 mU/L, 21.0± 4.8 mU/L, and 21.9 ± 6.6 mU/L, and compared with the control group, the blood insulin of the later two groups rises ( P< 0.05); then adding the NO-1886, at the end of the experiment the blood insulin in each group is 11.4 ± 6.2 mU/L, 20.4±2.3 mU/L, and 15.4± 1.8 mU/L; the content of insulin has no obvious difference between control group and NO-1886 group, while there is some difference between control group and sugar fat group ( P< 0.05) . Through insulin sensitive experiment, it was found that before injection blood insulin in each group is 19.3 ± 6.5 mU/L, 11.6 ±2.9mU/L, 19.3±7.1 mU/L, 30 min after injection the data is 123.6 ± 32.9 mU/L, 71.7 ±21.7 mU/L, and 141.5 ±29.4 mU/L, and 90 min after injection the data is 45.9±5.6 mU/L, 17.9± 12.4 mU/L, and 32.9± 12.9 mU/L, and compared with the control group, the content of insulin in the sugar fat group reduced (in the 30th min p< 0.05, in the 90th min P< 0.01). At the end of experiment, the pancreas' vanadium content in each group is0.8±0.8ng/g,0.7±0.1 ng/g, and 0.8 ± 0.3 ng/g, the chromium content is 2.3 ± 1.2 ng/g, 1.9± 0.6 ng/g, and 2.1 ± 0.9 ng/g, and compar with NO-1886 group, the vanadium and chromium content in the sugar fat group reduced( P< 0.05), while there is no obvious difference between control group and NO-1886 group. Conclusion NO-1886 can improve the content levels of vanadium and chromium and the sensitivity of the insulin in the pigs' pancreas with high fat and high sugar.