Aim To investigate whether antiphospholipid antibody increases the uptaking of oxidized low density lipoprotein and up regulates the expression of CD36 in U937 cells. Methods The cultured U937 cells were incubated with oxidized low density lipoprotein of 80 mg/L and antiphospholipid antibody of 100 mg/L for 48 hours in vitro. Then the contents of cholesterol and cholesterol ester were detected by enzyme fluorescence. The expression of CD36 on U937 was quantified by reverse transcription polymerase chain reaction (RT PCR) and flow cytometry respectively. Results In the present of oxidized low density lipoprotein, the uptaking of cholesterol and CD36 expression increased significantly after by U937 cells incubated with antiphospholipid antibody. Conclusion Antiphosphlipid antibody may accelerate atherosclerosis by up regulating CD36 expression.