Aim To observe whether the hyperplasia of pulmonary arteriole wall during pulmonary hypertension can be interfered by calcium channel-blocking. Methods Performed the anastomosis between left pulmonary artery and descending aorta in New Zealand rabbits, and banded the proximal left pulmonary artery of anastomosis, to establish the model of one-side kinetic pulmonary artery hypertension. After operation rabbits in drug group were fed with nifedipine and aspirin, and the blood concentration of endothelin and TXA2/PGI2 was measured regularly. Pulmonary artery pressure was measured and lung biopsy was performed in 2-3 months observation. Results During rabbits formatting pulmonary hypertension, those giving drugs got minor hyperplasia of pulmonary arteriole wall than those not giving drugs. The blood concentration of endothelin increased at all rabbits but the crest value was lower in drugs group. The ratio of TXA2/PGI2 was lower in drugs group too. Conclusion Calcium channel blocker can inhibit the hyperplasia of pulmonary arteriole wall during the formation of pulmonary hypertension, which could be the results that calcium channel blocking and aspirin inhibit the production of endothelin and TXA2 and promote the produce of PGI2.